Standard Operating Procedures

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separation line standard operating procedures

Please find here a selection of scientific papers related to Standard Operating Procedures (SOP). For Abstracts (if any), and Full Paper please follow corresponding links.


More Standard Operating Procedures at the Entire website


Sequential Targeting of CD52 and TNF Allows Early Minimization Therapy in Kidney Transplantation: From a Biomarker to Targeting in a Proof-Of-Concept Trial.

Viklicky O, Hruba P, Tomiuk S, Schmitz S, Gerstmayer B, Sawitzki B, Miqueu P, Mrazova P, Tycova I, Svobodova E, Honsova E, Janssen U, Volk HD, Reinke P.

PLoS One. 2017 Jan 13;12(1):e0169624. doi: 10.1371/journal.pone.0169624. eCollection 2017. PMID: 28085915 Free PMC Article

Age and gender leucocytes variances and references values generated using the standardized ONE-Study protocol.

Kverneland AH, Streitz M, Geissler E, Hutchinson J, Vogt K, Boës D, Niemann N, Pedersen AE, Schlickeiser S, Sawitzki B.

Cytometry A. 2016 Jun;89(6):543-64. doi: 10.1002/cyto.a.22855. Epub 2016 May 3. PMID: 27144459 Free Article

Differences in CD44 Surface Expression Levels and Function Discriminates IL-17 and IFN-γ Producing Helper T Cells.

Schumann J, Stanko K, Schliesser U, Appelt C, Sawitzki B.

PLoS One. 2015 Jul 14;10(7):e0132479. doi: 10.1371/journal.pone.0132479. eCollection 2015. Erratum in: PLoS One. 2015;10(11):e0143986. PMID: 26172046 Free PMC Article

Standardization of whole blood immune phenotype monitoring for clinical trials: panels and methods from the ONE study.

Streitz M, Miloud T, Kapinsky M, Reed MR, Magari R, Geissler EK, Hutchinson JA, Vogt K, Schlickeiser S, Kverneland AH, Meisel C, Volk HD, Sawitzki B.

Transplant Res. 2013 Oct 25;2(1):17. doi: 10.1186/2047-1440-2-17. PMID: 24160259 Free PMC Article

Allogeneic partially HLA-matched dendritic cells pulsed with autologous tumor cell lysate as a vaccine in metastatic renal cell cancer: a clinical phase I/II study.

Flörcken A, Kopp J, van Lessen A, Movassaghi K, Takvorian A, Jöhrens K, Möbs M, Schönemann C, Sawitzki B, Egerer K, Dörken B, Pezzutto A, Westermann J.

Hum Vaccin Immunother. 2013 Jun;9(6):1217-27. doi: 10.4161/hv.24149. Epub 2013 Mar 4. PMID: 23458999 Free PMC Article

Tregs: application for solid-organ transplantation.

Schliesser U, Streitz M, Sawitzki B.

Curr Opin Organ Transplant. 2012 Feb;17(1):34-41. doi: 10.1097/MOT.0b013e32834ee69f. Review.PMID: 22143395

Cutting Edge: Immunological consequences and trafficking of human regulatory macrophages administered to renal transplant recipients.

Hutchinson JA, Riquelme P, Sawitzki B, Tomiuk S, Miqueu P, Zuhayra M, Oberg HH, Pascher A, Lützen U, Janssen U, Broichhausen C, Renders L, Thaiss F, Scheuermann E, Henze E, Volk HD, Chatenoud L, Lechler RI, Wood KJ, Kabelitz D, Schlitt HJ, Geissler EK, Fändrich F.

J Immunol. 2011 Sep 1;187(5):2072-8. doi: 10.4049/jimmunol.1100762. Epub 2011 Jul 29. PMID: 21804023 Free Article

Allogeneic gene-modified tumor cells (RCC-26/IL-7/CD80) as a vaccine in patients with metastatic renal cell cancer: a clinical phase-I study.

Westermann J, Flörcken A, Willimsky G, van Lessen A, Kopp J, Takvorian A, Jöhrens K, Lukowsky A, Schönemann C, Sawitzki B, Pohla H, Frank R, Dörken B, Schendel DJ, Blankenstein T, Pezzutto A.

Gene Ther. 2011 Apr;18(4):354-63. doi: 10.1038/gt.2010.143. Epub 2010 Nov 11. PMID: 21068778

Alpha-1,2-mannosidase and hence N-glycosylation are required for regulatory T cell migration and allograft tolerance in mice.

Long ET, Baker S, Oliveira V, Sawitzki B, Wood KJ.

PLoS One. 2010 Jan 26;5(1):e8894. doi: 10.1371/journal.pone.0008894. PMID: 20126660 Free PMC Article

Guidelines for the use of flow cytometry and cell sorting in immunological studies.

Cossarizza A, Chang HD, Radbruch A, and >175 authors more

European Journal of Immunology. 2017 Oct;47(10):1584-1797. doi: 10.1002/eji.201646632.

Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study.

Duffy D, Rouilly V, Braudeau C, Corbière V, Djebali R, Ungeheuer MN, Josien R, LaBrie ST, Lantz O, Louis D, Martinez-Caceres E, Mascart F, Ruiz de Morales JG, Ottone C, Redjah L, Guen NS, Savenay A, Schmolz M, Toubert A, Albert ML; Multinational FOCIS Centers of Excellence.

Clininical Immunology2017 Oct;183:325-335. doi: 10.1016/j.clim.2017.09.019. Epub 2017 Sep 22.

Monitoring T cell responses in translational studies: optimization of dye-proliferation for evaluation of antigen-specific responses

Anja Ten Brinke*, Natalia Maria Marek- Trzonkowska, Maria Jose Mansilla, Annelies Turksma, Karolina Piekarska, Dorota Iwaszkiewicz-Grzes, Laura Passerini, Grazia Locafaro, Joan Puñet-Ortiz, S. Marieke Van Ham, Maria Puerto Hernandez-Fuentes, Eva Martinez-Caceres and Silvia Gregori

Frontiers in Immunology-Immunological Tolerance and Regulation, 2017 Dec .

Guidelines for the use of flow cytometry and cell sorting in immunological studies”

Cossarizza A, Chang HD, Radbruch A, et al

Eur Journal of Immunology. 2017 Oct; 47(10):1584-1797. doi: 10.1002/eji.201646632. PubMed PMID: 29023707.

Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study

Duffy D, Rouilly V, Braudeau C, Corbière V, Djebali R, Ungeheuer MN, Josien R, LaBrie ST, Lantz O, Louis D, Martinez-Caceres E, Mascart F, Ruiz de Morales JG,Ottone C, Redjah L, Guen NS, Savenay A, Schmolz M, Toubert A, Albert ML;

Multinational FOCIS Centers of Excellence. Clinical Immunology. 2017 Oct;183:325-335.

Virtual Global Transplant Laboratory Standard Operating Protocol for Donor Alloantigen-specific Interferon-gamma ELISPOT Assay.

Carroll R, Troelnikov A, Chong AS.

Transplant Direct. 2016 Oct 20;2(11):e111.

Virtual Global Transplant Laboratory Standard Operating Procedures for Blood Collection, PBMC Isolation, and Storage.

Higdon LE, Lee K, Tang Q, Maltzman JS.

Transplant Direct. 2016 Aug 18;2(9):e101.

First Publication of Standardized Immune Monitoring Methods From the Virtual Global Transplantation Laboratory Initiative.

Geissler EK.

Transplant Direct. 2016 Aug 18;2(9):e100. No abstract available.

Establishment of a global virtual laboratory for transplantation: a symposium report.

Geissler EK, Tullius SG, Chong AS.

Transplantation. 2015 Feb;99(2):381-4.

Standardization of whole blood immune phenotype monitoring for clinical trials:
panels and methods from the ONE study.

Streitz M, Miloud T, Kapinsky M, Reed MR, Magari R, Geissler EK, HutchinsonJA, Vogt K, Schlickeiser S, Kverneland AH, Meisel C, Volk HD, Sawitzki B.

Transplant Res. 2013 Oct 25;2(1):17. doi: 10.1186/2047-1440-2-17. PubMed PMID: 24160259; PubMed Central PMCID: PMC3827923.4