Joint AFACTT papers

Share itShare on FacebookTweet about this on TwitterShare on LinkedInShare on Google+Print this pageEmail this to someone

separation line joint afactt papers

Please find here a selection of scientific papers produced as a direct result of the AFACTT project and written by (one or more of) its members. Papers are grouped into those awaiting publication and those already published. Papers in OPEN ACCESS carry a link.


The following papers are either in press, accepted but not published yet or submittted (January 2018)


Cell Banking For Regulatory T Cell- Based Therapy: Strategies To Overcome the Impact of Cryopreservation On the Treg Viability And Phenotype.

Karolina Golab, Randall Grose, Veronica Placencia, Amittha Wickrema, Julia Solomina, Martin Tibudan, Evelyn Konsur, Kamil Cichy, Natalia Marek-Trzonkowska, Piotr Trzonkowski, J. Michael Millis, John Fung, Piotr Witkowski.

Oncotarget 2017 in press

Engineered T regulatory type 1 (Tr1) cells for clinical applications.

Gregori S and Roncarolo MG.

Front Immunol 2017 ( in press)

Targeting trained immunity promotes organ transplant acceptance.

Braza MS, Lameijer M, Sanchez-Gaytan B, Arts R, Pérez-Medina C, Conde P, Brahmachary M, van der Touw W, Fay F, Kluza E, Kossatz S, Stroes E, Kroon J, Dress R, Salem F, Rialdi A, Reiner T, Boros P, van Leent M, Strijkers G, Calcagno C, Ginhoux F, Marazzi I, Lutgens E, Nicolaes G, Weber C, Swirski F, Nahrendorf M, Fisher E, Fayad Z, Duivenvoorden R, Netea M, Ochando J, and Mulder WJ.
(Manuscript Submitted)

Neutrophil derived CSF1 induces macrophage polarization and promotes transplantation tolerance.

Braza MS, Conde P, Garcia MR, Cortegano I, Brahmachary M, Pothula V, Fay F, Boros P, Werner SW, Ginhoux F, Mulder WJ, and Ochando J.

Am J Transplant. 2017. Accepted.

Minimum information about T regulatory cells (MITREG): a step towards reproducibility and standardisation

Running title: Minimum information about T regulatory cells Front Immunology 2017 (in press)

Anke Fuchs, Mateusz Gliwinski; Nathali Grageda, Rachel Spiering, Abul K. Abbas , Silke Appel, Rosa Bacchetta, Manuela Battaglia, David Berglund, Bruce Blazar, Jeffrey 7 A. Bluestone, Martin Bornh¨a;user, Anja ten Brinke, Todd M. Brusko, Nathalie Cools, Maria Cristina Cuturi, Edward Geissler, Nick Giannoukakis, Karolina Golab, David A. Hafler, Marieke van Ham, Joanna Hester, Keli Hippen, Mauro Di Ianni, Natasa Ilic, John Isaacs, Fadi Issa, Dorota Iwaszkiewicz-Grzes, Elmar Jaeckel, Irma Joosten, David Klatzmann, Hans Koenen, Cees van Kooten, Olle Korsgren, Karsten Kretschmer, Megan Levings, Natalia-MarekTrzonkowska,Marc Martinez-Llordella, 13 Djordje Miljkovic, Kingston H.G.Mills, Joana P Miranda, Ciriaco A. Piccirillo, Amy L Putnam , Thomas Ritter, Maria Grazia Roncarolo, Shimon Sakaguchi, Silvia Sánchez-Ramón, Birgit Sawitzki, Ljiljana Sofronic-Milosavljevic, Megan Sykes, Qizhi Tang, Marta Vives-Pi, Herman Waldmann, Piotr Witkowski, Kathryn J.Wood, Silvia Gregori, Catharien M.U. Hilkens, Giovanna Lombardi, Phillip Lord, Eva M Martinez-Caceres, Piotr Trzonkowski

Monitoring T cell responses in translational studies: optimization of dyeproliferation for evaluation of antigen-specific responses.

Anja Ten Brinke, Natalia M. Marek- Trzonkowska, Maria Jose Mansilla, Annelies Turksma, Karolina Piekarska, Dorota Iwaszkiewicz-Grzes, Laura Passerini, Grazia Locafaro, Joan Puñet-Ortiz, Marieke Van Ham, Maria P. Hernandez-Fuentes, Eva Martinez Caceres , Silvia Gregori.

Front Immunology 2017, in press

Generation of TIGIT+ Tregs by Human Regulatory Macrophages Before Kidney Transplantation (submitted to Nat Commun)

Paloma Riquelme, Jan Haarer, Anja Kammler, Lisa Walter, Stefan Tomiuk, Norbert Ahrens, Ivan Goecze, Anja K. Wege, Daniel Zecher, Fred Fändrich, Hans J. Schlitt, Bernhard Banas, Manfred B. Lutz, Tuija Kekarainen, Birgit Sawitzki, Jordi Ochando, Edward K. Geissler and James A. Hutchinson1.


The following papers have been published. Papers in OPEN ACCESS contain a link


T follicular helper cells: a potential therapeutic target in follicular lymphoma.

Ochando J and Braza MS.

Oncotarget 2017.

DOI10.18632/oncotarget.22788

Nanoparticle-based modulation and monitoring of antigenpresenting cells in organ transplantation.

Ochando J and Braza MS.

Front Immunol. 2017.

DOI:10.3389/fimmu.2017.01888

Patients with Primary Sjögren’s Syndrome Have Alterations in Absolute Quantities of Specific Peripheral Leucocyte Populations.

Davies R, Hammenfors D, Bergum B, Jakobsen K, Solheim M, Vogelsang P, Brun JG, Bryceson Y, Jonsson R, Appel S.

Scand J Immunol. 2017 Dec;86(6):491-502.

doi: 10.1111/sji.12622. PubMed PMID: 29072325.

Islet cell transplant and the incorporation of Tregs.

Krzystyniak A, Golab K, Witkowski P, Trzonkowski P.

Curr Opin Organ Transplant. 2014 Dec;19(6):610-5.

doi: 10.1097/

CD45RA Distinguishes CD4+CD25+CD127-/low TSDR Demethylated Regulatory T Cell Subpopulations With Differential Stability and Susceptibility to Tacrolimus-Mediated Inhibition of Suppression.

Arroyo Hornero R, Betts GJ, Sawitzki B, Vogt K, Harden PN, Wood KJ.

Transplantation. 2017 Feb;101(2):302-309.

doi: 10.1097/TP.0000000000001278. PubMed PMID: 28118317; PubMed Central PMCID: PMC5265687.

Clinical Outlook for Type-1 and FOXP3(+) T Regulatory Cell-Based Therapy.

Gregori S, Passerini L, Roncarolo MG.

Front Immunol. 2015 Nov 25;6:593.

doi: 10.3389/fimmu.2015.00593.

eCollection 2015. Review. PubMed PMID: 26635807; PubMed Central PMCID: PMC4658444.

HLA-G expression levels influence the tolerogenic activity of human DC-10.

Amodio G, Comi M, Tomasoni D, Gianolini ME, Rizzo R, LeMaoult J, Roncarolo MG, Gregori S.

Haematologica. 2015 Apr;100(4):548-57.

doi: 10.3324/haematol.2014.113803.

Epub 2015 Feb 6. PubMed PMID: 25661445; PubMed Central PMCID: PMC4380729.

Factors affecting long-term efficacy of T regulatory cell-based therapy in type 1 diabetes.

Marek-Trzonkowska N, Mysliwiec M, Iwaszkiewicz-Grzes D, Gliwinski M, Derkowska I, Zalinska M, Zielinski M, Grabowska M, Zielinska H, Piekarska K, Jazwinska-Curyllo A, Owczuk R, Szadkowska A, Wyka K, Witkowski P, Mlynarski W, Jarosz-Chobot P, Bossowski A, Siebert J, Trzonkowski P.

J Transl Med. 2016 Dec 1;14(1):332.

PubMed PMID: 27903296; PubMed Central PMCID: PMC5131539.

Mild hypothermia provides Treg stability.

Marek- Trzonkowska N, Piekarska K, Filipowicz N, Piotrowski A, Gucwa M, Vogt K, Sawitzki B, Siebert J, Trzonkowski P.

Sci Rep. 2017; 7: 11915

doi: 10.1038/s41598-017-10151-

Cell-based Tolerogenic Therapy, Experience from Animal Models of Multiple Sclerosis, Type 1 Diabetes and Rheumatoid Arthritis.

Stojanovic I, Dimitrijevic M, Vives-Pi M, Mansilla MJ, Pujol-Autonell I, Rodríguez-Fernandez S, Palova-Jelínkova L, Funda DP, Gruden-Movsesijan A,
Sofronic-Milosavljevic L, Hilkens CMU, Martinez-Caceres E, Miljkovic D.

Curr Pharm Des. 2017;23(18):2623-2643.

doi: 10.2174/1381612823666170214120708. PubMed PMID: 28201972.

Immunomodulatory Effects of 1,25-Dihydroxyvitamin D(3) on Dendritic Cells Promote Induction of T Cell Hyporesponsiveness to Myelin-Derived Antigens.

Lee WP, Willekens B, Cras P, Goossens H, Martínez-Cáceres E, Berneman ZN, Cools N.

J Immunol Res. 2016;2016:5392623.

Epub 2016 Sep 14. PubMed PMID: 27703987; PubMed Central PMCID: PMC5039280.

Minimum information about tolerogenic antigen-presenting cells (MITAP): a first step towards reproducibility and standardisation of cellular therapies.

Lord P, Spiering R, Aguillon JC, Anderson AE, Appel S, Benitez-Ribas D, Ten Brinke A, Broere F, Cools N, Cuturi MC, Diboll J, Geissler EK, Giannoukakis N, Gregori S, van Ham SM, Lattimer S, Marshall L, Harry RA, Hutchinson JA, Isaacs JD, Joosten I, van Kooten C, Lopez Diaz de Cerio A, Nikolic T, Oral HB, Sofronic-Milosavljevic L, Ritter T, Riquelme P, Thomson AW, Trucco M, Vives-Pi M, Martinez-Caceres EM, Hilkens CMU.

PeerJ. 2016 Aug 30;4:e2300.

doi: 10.7717/peerj.2300. eCollection 2016. PubMed PMID: 27635311; PubMed Central PMCID: PMC5012269.

Cryopreserved vitamin D3-tolerogenic dendritic cells pulsed with autoantigens as a potential therapy for multiple sclerosis patients.

Mansilla MJ, Contreras-Cardone R, Navarro-Barriuso J, Cools N, Berneman Z, Ramo-Tello C, Martínez-Cáceres EM.

J Neuroinflammation. 2016 May 20;13(1):113.

doi: 10.1186/s12974-016-0584-9. PubMed PMID: 27207486; PubMed Central PMCID: PMC4874005.

Clinical Use of Tolerogenic Dendritic Cells-Harmonization Approach in European Collaborative Effort.

Ten Brinke A, Hilkens CM, Cools N, Geissler EK, Hutchinson JA, Lombardi G, Lord P, Sawitzki B, Trzonkowski P, Van Ham SM, Martinez-Caceres EM.

Mediators Inflamm. 2015;2015:471719.

doi: 10.1155/2015/471719.

Epub 2015 Dec 24. Review. PubMed PMID: 26819498; PubMed Central PMCID: PMC4706930.

Hurdles in therapy with regulatory T cells.

Trzonkowski P, Bacchetta R, Battaglia M, Berglund D, Bohnenkamp HR, ten Brinke A, Bushell A, Cools N, Geissler EK, Gregori S, Marieke van Ham S, Hilkens C, Hutchinson JA, Lombardi G, Madrigal JA, Marek-Trzonkowska N, Martinez-Caceres EM, Roncarolo MG, Sanchez-Ramon S, Saudemont A, Sawitzki B.

Sci Transl Med. 2015 Sep 9;7(304):304ps18.

doi: 10.1126/scitranslmed.aaa7721. Review. PubMed PMID: 26355029.

DC-SIGN(+) Macrophages Control the Induction of Transplantation Tolerance.

Conde P, Rodriguez M, van der Touw W, Jimenez A, Burns M, Miller J, Brahmachary M, Chen HM, Boros P, Rausell-Palamos F, Yun TJ, Riquelme P, Rastrojo A, Aguado B, Stein-Streilein J, Tanaka M, Zhou L, Zhang J, Lowary TL, Ginhoux F, Park CG, Cheong C, Brody J, Turley SJ, Lira SA, Bronte V, Gordon S, Heeger PS, Merad M, Hutchinson J, Chen SH, Ochando J.

Immunity. 2015 Jun 16;42(6):1143-58.

doi: 10.1016/j.immuni.2015.05.009.

Epub 2015 Jun 9. PubMed PMID: 26070485; PubMed Central PMCID: PMC4690204.

Targeting of tolerogenic dendritic cells towards heat-shock proteins: a novel therapeutic strategy for autoimmune diseases?

Jansen MAA, Spiering R, Broere F, van Laar JM, Isaacs JD, van Eden W, Hilkens CMU.

Immunology. 2018 Jan;153(1):51-59.

doi: 10.1111/imm.12811.

Epub 2017 Sep 18. Review. PubMed PMID: 28804903; PubMed Central PMCID: PMC5721256.

The role of epigenetic modifiers in extended cultures of functional hepatocyte-like cells derived from human neonatal mesenchymal stem cells.

Cipriano M, Freyer N, Knöspel F, Oliveira NG, Barcia R, Cruz PE, Cruz H, Castro M, Santos JM, Zeilinger K, Miranda JP. Cipriano M, Correia JC, Camões SP, Oliveira NG, Cruz P, Cruz H, Castro M, Ruas JL, Santos JM, Miranda JP.

Arch Toxicol. 2017 Jun;91(6):2469-2489.

doi: 10.1007/s00204-016-1901-x.

Epub 2016 Dec 1. PubMed PMID: 27909741.

Self-assembled 3D spheroids and hollow-fibre bioreactors improve MSC-derived hepatocyte-like cell maturation in vitro.

Cipriano M, Freyer N, Knöspel F, Oliveira NG, Barcia R, Cruz PE, Cruz H, Castro M, Santos JM, Zeilinger K, Miranda JP.

Arch Toxicol. 2017 Apr;91(4):1815-1832.

doi: 10.1007/s00204-016-1838-0.

Epub 2016 Sep 2. PubMed PMID: 27590069. doi: 10.1007/s00204-016-1838-

IL-10-Engineered Human CD4(+) Tr1 Cells Eliminate Myeloid Leukemia in an HLA Class I-Dependent Mechanism.

Locafaro G, Andolfi G, Russo F, Cesana L, Spinelli A, Camisa B, Ciceri F, Lombardo A, Bondanza A, Roncarolo MG, Gregori S.

Mol Ther. 2017 Oct 4;25(10):2254-2269.

doi: 10.1016/j.ymthe.2017.06.029.

Epub 2017 Jul 5. PubMed PMID: 28807569; PubMed Central PMCID: PMC5628869. doi: 10.1016/j.ymthe.2017.06.0